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Lab Tests Explained Part 5: Urine Routine Test – Protein, Sugar, Pus Cells and What They Mean

A simple urine test can reveal infections, kidney problems and diabetes. Here is how to read each line of your urine routine report and how to collect the sample correctly.

Laboratory counter with sterile urine sample containers, test strips and a microscope
Laboratory counter with sterile urine sample containers, test strips and a microscope
In this article

    The urine routine and microscopy test is quick, cheap and surprisingly informative. It can point to urinary infections, kidney disease, diabetes, dehydration and even liver problems. Here is how to make sense of the report.

    How to give a good sample

    • Use the sterile container from the lab and do not touch the inside.
    • Wash the genital area with water first.
    • Collect a midstream sample: pass the first part of the urine into the toilet, then collect the middle portion.
    • A first-morning sample is often preferred, and it should reach the lab within an hour or two.
    • Women should ideally avoid testing during menstruation, as blood can contaminate the sample.

    Physical examination

    ParameterUsual findingWhat changes may mean
    ColourPale to dark yellowVery dark: dehydration or bilirubin; red or brown: blood, certain foods or medicines
    AppearanceClearCloudy: infection, crystals or mucus
    Specific gravityabout 1.005 – 1.030High: dehydration; persistently low: kidney concentrating problems
    pHabout 4.5 – 8.0Can be influenced by diet, infection and stone risk

    Chemical examination

    • Protein (albumin): normally nil or trace. Persistent protein can signal kidney damage, often from diabetes or high blood pressure. Fever, heavy exercise and standing for long periods can cause temporary traces.
    • Glucose (sugar): normally nil. Sugar in urine usually means blood sugar is high, and diabetes should be checked.
    • Ketones: normally nil. They appear with fasting, very low-carb diets, vomiting or, more seriously, uncontrolled diabetes.
    • Bilirubin and urobilinogen: changes can point to liver or bile duct problems.
    • Nitrite and leukocyte esterase: positive results suggest a bacterial urinary tract infection.
    • Blood: normally negative; see the RBC count below.

    Microscopic examination

    FindingUsual rangePossible meaning if raised
    Pus cells (WBCs)0 – 5 per high-power fieldUrinary tract infection or inflammation
    Red blood cells0 – 2 per high-power fieldStones, infection, injury, kidney disease; needs follow-up
    Epithelial cellsFewMany: often a contaminated sample
    CastsNone or occasional hyalineSome types suggest kidney disease
    CrystalsOccasionalMany: stone risk, diet or dehydration
    BacteriaNil or fewInfection or contamination

    Urinary tract infection (UTI) clues

    Burning while passing urine, frequent urge, lower abdominal pain or fever, together with raised pus cells and positive nitrite, suggest a UTI. Your doctor may order a urine culture to identify the bacteria and choose the right antibiotic. Avoid taking leftover antibiotics on your own, which can fuel resistance.

    When to see a doctor

    Consult a doctor if your report shows protein, sugar, blood or many pus cells, or if you have pain, fever, blood in the urine, swelling of the feet or face, or a sudden change in how much you urinate.

    Lab Tests Explained: the full series

    Related reads: Blood sugar levels and HbA1c · Thyroid tests · Vitamin D test · Uric acid

    Disclaimer: This article is for general information only and is not medical advice. Reference ranges vary between laboratories, so always read your report against the range printed on it and discuss results with a qualified doctor.

    Written by Raj

    Founder & Editor

    I write HorizonDesk: practical, no-hype articles on technology, money, business and the vehicles we drive. Everything here is something I’ve researched, tested or wanted explained clearly myself.